Background & Objectives: brucellosis is a zoonosis and has remained a major health and economic concern. The main strategies for control and prevention of brucellosis include Intersectoral collaboration, disease surveillance, community education, and training of health workers. The aim of this study was to evaluate the effectiveness of educational interventions on reduction of brucellosis in rural areas of Khomein city. Methods: In a randomized semi-experimental study 400 adult men from rural areas of Khomein city were randomly allocated to case and control groups. Data were gathered using a researcher designed questionnaire based on PRECEDE framework. Educational interventions were conducted in three sessions (one session in a week) for 60 to 90 minutes based on the PRECEDE framework and the subjects were followed for 3 months. Results: Mean scores of predisposing factors (knowledge and attitude), reinforcing and enabling factors showed significant differences between case and control groups after intervention (p<0.05). Brucellosis incidence reduced from 147 in 2006 to 43 cases per 100000 in the first nine mounts of 2008 (p<0.05). Conclusion: Planning and implementing of health education interventions based on PRECEDE framework could be effective on reduction of brucellosis incidence.