[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Flowchart of approve and expert process::
indexing and abstracting::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 14, Issue 4 (winter 2024) ::
j.health 2024, 14(4): 402-411 Back to browse issues page
Investigation of Coronavirus RNA in Hospital Wastewater and Municipal Wastewater Treatment Plants in Ardabil Province
A Dargahi , M Vosoughi , H Gholizadeh , Ch Karami , H* Sadeghi * , A Normohammadi , F Jeddi
Ardabil University of Medical Sciences, Ardabil, Iran
Abstract:   (312 Views)
Background & objectives: Since 2019, the spread of the coronavirus has caused an epidemic worldwide, and the transmission of the disease through respiratory droplets has been declared as the primary method of transmission in 2020. However, in this study, the indirect transmission of the virus through sewage has been investigated.
Methodology: Wastewater related to hospital and urban wastewater treatment plants in 5 regions of Ardabil province (northwest of Iran) were investigated. A total of 22 samples were kept in pre-test conditions (temperature -20 degrees Celsius) and were used to identify the viral genome. Specific primer and chain reaction probes targeting ORF1ab and N (nucleoprotein gene) genes were used.
Results: In this research, out of all the samples collected, the result of 3 samples was positive. All three positive samples are related to incoming sewage.
Conclusion: The results of this research showed that sewage epidemiology (WBE) can be considered a cost-effective method for diagnosing and predicting pathogenic agents and an effective method for making decisions to protect citizens' health.
 
Keywords: SARS-CoV-2, Hospital Wastewater, Municipal Wastewater, Coronavirus, Wastewater Treatment Plant, Collection Network
Full-Text [PDF 804 kb]   (203 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/06/4 | Accepted: 2024/01/13 | Published: 2024/01/13
References
1. A Alygizakis N, Markou AN, Rousis NI, Galani A, Avgeris M, Adamopoulos PG, Scorilas A, Lianidou ES, Paraskevis D, Tsiodras S, Tsakris A. nalytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives. TrAC Trends in Analytical Chemistry. 2021;134:116125. [DOI:10.1016/j.trac.2020.116125] [PMID] []
2. Chekol WB, Melesse DY. Operating room team safety and perioperative anesthetic management of patients with suspected or confirmed novel corona virus in resource limited settings: A systematic review. Trends in Anaesthesia and Critical Care. 2020;34:14-22. [DOI:10.1016/j.tacc.2020.06.011] []
3. Amoah ID, Kumari S, Bux F. Coronaviruses in wastewater processes: source, fate and potential risks. Environment international. 2020;143:105962. [DOI:10.1016/j.envint.2020.105962] [PMID] []
4. Lai C-C, Ko W-C, Lee P-I, Jean S-S, Hsueh P-R. Extra-respiratory manifestations of COVID-19. International journal of antimicrobial agents. 2020;56(2):106024. [DOI:10.1016/j.ijantimicag.2020.106024] [PMID] []
5. Asghar H, Diop OM, Weldegebriel G, Malik F, Shetty S, El Bassioni L, et al. Environmental surveillance for polioviruses in the Global Polio Eradication Initiative. The Journal of infectious diseases. 2014;210(suppl_1):S294-S303. [DOI:10.1093/infdis/jiu384] [PMID] []
6. Han J, He S. Urban flooding events pose risks of virus spread during the novel coronavirus (COVID-19) pandemic. Science of the Total Environment. 2021;755:142491. [DOI:10.1016/j.scitotenv.2020.142491] [PMID] []
7. Jiehao C, Jin X, Daojiong L, Zhi Y, Lei X, Zhenghai Q, et al. A case series of children with 2019 novel coronavirus infection: clinical and epidemiological features. Clinical Infectious Diseases. 2020;71(6):1547-51. [DOI:10.1093/cid/ciaa198] [PMID] []
8. Shaldam MA, Yahya G, Mohamed NH, Abdel-Daim MM, Al Naggar Y. In silico screening of potent bioactive compounds from honeybee products against COVID-19 target enzymes. Environmental Science and Pollution Research. 2021;28(30):40507-14. [DOI:10.1007/s11356-021-14195-9] [PMID] []
9. Michael-Kordatou I, Karaolia P, Fatta-Kassinos D. Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification. Journal of environmental chemical engineering. 2020;8(5):104306. [DOI:10.1016/j.jece.2020.104306] [PMID] []
10. Vosoughi M, Karami C, Dargahi A, Jeddi F, Jalali KM, Hadisi A, et al. Investigation of SARS-CoV-2 in hospital indoor air of COVID-19 patients' ward with impinger method. Environmental Science and Pollution Research. 2021;28(36):50480-8. [DOI:10.1007/s11356-021-14260-3] [PMID] []
11. Mallapaty S. How sewage could reveal true scale of coronavirus outbreak. Nature. 2020;580(7802):176-7. [DOI:10.1038/d41586-020-00973-x] [PMID]
12. Lahrich S, Laghrib F, Farahi A, Bakasse M, Saqrane S, El Mhammedi M. Review on the contamination of wastewater by COVID-19 virus: Impact and treatment. Science of The Total Environment. 2021;751:142325. [DOI:10.1016/j.scitotenv.2020.142325] [PMID] []
13. Carducci A, Federigi I, Liu D, Thompson JR, Verani M. Making waves: coronavirus detection, presence and persistence in the water environment: state of the art and knowledge needs for public health. Water research. 2020;179:115907. [DOI:10.1016/j.watres.2020.115907] [PMID] []
14. Waggoner JJ, Stittleburg V, Pond R, Saklawi Y, Sahoo MK, Babiker A, et al. Triplex real-time RT-PCR for severe acute respiratory syndrome coronavirus 2. Emerging infectious diseases. 2020;26(7):1633. [DOI:10.3201/eid2607.201285] [PMID] []
15. Wilson AM, Sleeth DK, Schaefer C, Jones RM. Transmission of respiratory viral diseases to health care workers: COVID-19 as an example. Annual Review of Public Health. 2022;43:311-30. [DOI:10.1146/annurev-publhealth-052120-110009] [PMID]
16. Manoj M, Kumar MS, Valsaraj K, Sivan C, Vijayan SK. Potential link between compromised air quality and transmission of the novel corona virus (SARS-CoV-2) in affected areas. Environmental research. 2020;190:110001. [DOI:10.1016/j.envres.2020.110001] [PMID] []
17. Lodder W, de Roda Husman AM. SARS-CoV-2 in wastewater: potential health risk, but also data source. The Lancet Gastroenterology & Hepatology. 2020;5(6):533-4. [DOI:10.1016/S2468-1253(20)30087-X] [PMID]
18. Gao QY, Chen YX, Fang JY. 2019 Novel coronavirus infection and gastrointestinal tract. Journal of digestive diseases. 2020;21(3):125. [DOI:10.1111/1751-2980.12851] [PMID] []
19. Holshue ML, DeBolt C, Lindquist S, Lofy KH, Wiesman J, Bruce H, et al. First case of 2019 novel coronavirus in the United States. New England journal of medicine. 2020;382(10):929-36. [DOI:10.1056/NEJMoa2001191] [PMID] []
20. Xagoraraki I, O'Brien E. Wastewater-based epidemiology for early detection of viral outbreaks. Women in water quality: Investigations by prominent female engineers. 2020:75-97. [DOI:10.1007/978-3-030-17819-2_5] []
21. Sen-Crowe B, Boneva D, Elkbuli A. Municipal sewage COVID-19 testing: a much needed public health community prevention intervention. The American Surgeon. 2020;86(11):1518-9. [DOI:10.1177/0003134820951428] [PMID]
22. Hellmér M, Paxéus N, Magnius L, Enache L, Arnholm B, Johansson A, et al. Detection of pathogenic viruses in sewage provided early warnings of hepatitis A virus and norovirus outbreaks. Applied and environmental microbiology. 2014;80(21):6771-81. [DOI:10.1128/AEM.01981-14] [PMID] []
23. Hillary LS, Malham SK, McDonald JE, Jones DL. Wastewater and public health: the potential of wastewater surveillance for monitoring COVID-19. Current Opinion in Environmental Science & Health. 2020;17:14-20. [DOI:10.1016/j.coesh.2020.06.001] [PMID] []
24. Medema G, Heijnen L, Elsinga G, Italiaander R, Brouwer A. Presence of SARS-Coronavirus-2 RNA in sewage and correlation with reported COVID-19 prevalence in the early stage of the epidemic in the Netherlands. Environmental Science & Technology Letters. 2020;7(7):511-6. [DOI:10.1021/acs.estlett.0c00357] [PMID]
25. Wu F, Zhang J, Xiao A, Gu X, Lee WL, Armas F, et al. SARS-CoV-2 titers in wastewater are higher than expected from clinically confirmed cases. Msystems. 2020;5(4):10.1128/msystems. 00614-20. [DOI:10.1128/mSystems.00614-20] [PMID] []
26. Bhowmick GD, Dhar D, Nath D, Ghangrekar MM, Banerjee R, Das S, et al. Coronavirus disease 2019 (COVID-19) outbreak: some serious consequences with urban and rural water cycle. NPJ Clean Water. 2020;3(1):32. [DOI:10.1038/s41545-020-0079-1]
27. Lai C-C, Shih T-P, Ko W-C, Tang H-J, Hsueh P-R. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges. International journal of antimicrobial agents. 2020;55(3):105924. [DOI:10.1016/j.ijantimicag.2020.105924] [PMID] []
28. Martínez-Puchol S, Rusiñol M, Fernández-Cassi X, Timoneda N, Itarte M, Andrés C, et al. Characterisation of the sewage virome: comparison of NGS tools and occurrence of significant pathogens. Science of the Total Environment. 2020;713:136604. [DOI:10.1016/j.scitotenv.2020.136604] [PMID]
29. Martínez-Puchol S, Itarte M, Rusiñol M, Forés E, Mejías-Molina C, Andrés C, et al. Exploring the diversity of coronavirus in sewage during COVID-19 pandemic: Don't miss the forest for the trees. Science of the Total Environment. 2021;800:149562. [DOI:10.1016/j.scitotenv.2021.149562] [PMID] []
30. Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, Müller MA, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020;581(7809):465-9. [DOI:10.1038/s41586-020-2196-x] [PMID]
31. Lesté-Lasserre C. Coronavirus found in Paris sewage points to early warning system. Science. 2020. [DOI:10.1126/science.abc3799]
32. Yuan J, Chen Z, Gong C, Liu H, Li B, Li K, et al. Sewage as a possible transmission vehicle during a coronavirus disease 2019 outbreak in a densely populated community: Guangzhou, China, April 2020. Clinical Infectious Diseases. 2021;73(7):e1795-e802. [DOI:10.1093/cid/ciaa1494] [PMID] []
33. Elsamadony M, Fujii M, Miura T, Watanabe T. Possible transmission of viruses from contaminated human feces and sewage: Implications for SARS-CoV-2. Science of the Total Environment. 2021;755:142575. [DOI:10.1016/j.scitotenv.2020.142575] [PMID] []
34. Ali M, Zaid M, Saqib MAN, Ahmed H, Afzal MS. SARS‐CoV‐2 and the hidden carriers: Sewage, feline, and blood transfusion. Journal of Medical Virology. 2020;92(11):2291. [DOI:10.1002/jmv.25956] [PMID] []
35. Mandal P, Gupta AK, Dubey BK. A review on presence, survival, disinfection/removal methods of coronavirus in wastewater and progress of wastewater-based epidemiology. Journal of environmental chemical engineering. 2020;8(5):104317. [DOI:10.1016/j.jece.2020.104317] [PMID] []
36. Kumar M, Alamin M, Kuroda K, Dhangar K, Hata A, Yamaguchi H, et al. Potential discharge, attenuation and exposure risk of SARS-CoV-2 in natural water bodies receiving treated wastewater. npj clean Water. 2021;4(1):8. [DOI:10.1038/s41545-021-00098-2]
37. Corman V, Bleicker T, Brünink S, Drosten C, Zambon M. Diagnostic detection of 2019-nCoV by real-time RT-PCR. World Health Organization. 2020;17:1-13.
38. Bar-Or I, Yaniv K, Shagan M, Ozer E, Weil M, Indenbaum V, et al. Regressing SARS-CoV-2 sewage measurements onto COVID-19 burden in the population: a proof-of-concept for quantitative environmental surveillance. Frontiers in Public Health. 2022;9:561710. [DOI:10.3389/fpubh.2021.561710] [PMID] []
39. Napit R, Manandhar P, Chaudhary A, Shrestha B, Poudel A, Raut R, et al. Rapid genomic surveillance of SARS-CoV-2 in a dense urban community of Kathmandu Valley using sewage samples. PLoS One. 2023;18(3):e0283664. [DOI:10.1371/journal.pone.0283664] [PMID] []
40. Wang W, Xu Y, Gao R, Lu R, Han K, Wu G, et al. Detection of SARS-CoV-2 in different types of clinical specimens. Jama. 2020;323(18):1843-4. [DOI:10.1001/jama.2020.3786]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dargahi A, Vosoughi M, Gholizadeh H, Karami C, Sadeghi H, Normohammadi A et al . Investigation of Coronavirus RNA in Hospital Wastewater and Municipal Wastewater Treatment Plants in Ardabil Province. j.health 2024; 14 (4) :402-411
URL: http://healthjournal.arums.ac.ir/article-1-2827-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 4 (winter 2024) Back to browse issues page
مجله سلامت و بهداشت Journal of Health
Persian site map - English site map - Created in 0.15 seconds with 37 queries by YEKTAWEB 4623