Background & Objectives: Ag nanoparticles is an effective option for water and wastewater disinfection due to their high antimicrobial properties. Application of AgNPs was limited because of difficulties associated with their separation from aqueous environments. In this study, AgNPs were coated on Fe3O4 nano particles to achieve magnetic separation of AgNPs from solution after disinfection process. Methods: In this study composites of magnetic silver nanocomposites and the one modified with carbon layer have been synthesized and their disinfection potentials on FC were tested through direct fermentation tube method. Efficiencies of two disinfectants were compared using nonparametric Wilcoxon test. Effects of disinfectants concentrations (0-60 mg/L), initial MPN of bacteria (101-105 MPN/100mL), and contact times (0-60 min), as well as the reusability of disinfectant (1-7 rounds) were studied. Results: Efficiency of Fe3O4@C@Ag nanoparticles significantly differed from Fe3O4@Ag (p<0.05) based on Wilcoxon test result. The standard discharge conditions were achieved at 40 mg/L of Fe3O4@C@Ag after 30 minutes contact time. Reuse of Fe3O4@C@Ag showed that this disinfectant can be reused 7 rounds without significant decrease in their efficiency. The effluent analysis revealed that at different pH levels silver ion concentrations in effluent were lower than the standard level even after 24 hours. Conclusion: Nanocomposite of Fe3O4@C@Ag has greate potential to be used in urban wastewater treatment due to high disinfection efficiency, reusability, production of no hazardious byproduct.