Background & Objectives: Education is the most powerful tool for transferring knowledge to people. Retraining course is one of the different education methods. It is important and worthful to train techniques of clinical breast exam, related skills, and new exam methods in order to increase midwifes’ knowledge about their tasks and responsibilities and develop skills and abilities to cope with new situations. Methods: A cross-sectional study was done in health centers of Ardabil city. All midwifes working in Ardabil health centers were studied before and after retraining using a questionnaire made based on retraining content. Data were analyzed using SPSS 12. Results: There was statistically significant differences between knowledge scores for before and after education (p<0.05) showing positive effect of retraining. Results showed that the age of subjects studied was on average 30.1 years (30-34) and individual study was the most important resources for information obtained (20 percent). However, 45 percent of midwifes had no tainting courses so far. Conclusion: Considering the remarkable effect of short term retraining courses on midwifes knowledge, holding these kinds of courses for all employed midwifes in health centers is necessary.