Background & objectives: The group A human Rotaviruses are the most important agents causing acute diarrhea in children worldwide. Rotaviruses are widely present in the environmental waters and are a serious risk for public health. This study was aimed to assess efficiency of group A human Rotaviruses removal from the concentrated sewage of Ghods and Mahallati wastewater treatment systems in Tehran using ELIZA method.

Methods: This cross-sectional study was carried out on 92 sewages samples collected from both influent and effluent system of Ghods and Mahallati sewage disposal systems by using grab sampling method.  All samples were concentrated by using three concentration methods: Pellet, Two-phase and Dex-PEG. Then Group A human Rotaviruses were identified with Enzyme Immunoassay (EIA) method.

Results: In total, rotaviruses were identified in 30 samples (32.61%). Rotaviruses were found in 35.48 and 26.67% of influent and effluent samples, respectively. The efficiencies of wastewater plants were 69.23 and 55.55% in Ghodas and Mahallati refining systems, respectively.

Conclusion: This study showed that the evaluated sewage disposal systems don’t have necessary efficiency to completely elimination Group A human Rotaviruses. Therefore, it is necessary to monitor constantly the wastewater treatment plant to revise water pollution indicators and to seek new methods of wastewater treatment methods for complete removing of human viruses.