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:: Volume 5, Issue 3 (autumn 2014) ::
j.health 2014, 5(3): 224-234 Back to browse issues page
Extraction and Determination of Vancomycin Antibiotic in Hospital Sewage Samples by Solid Phase Extraction-High Performance Liquid Chromatography
SN Safavy , SJ Ghaemmaghami Hezaveh , R Dehghanzadeh Rayhani
Tabriz University of Medical Sciences
Abstract:   (5416 Views)

Background & Objectives: Vancomycin, as β-lactam classes of antibiotics, is widely used for treating infectious diseases in hospitals. Therefore, it is necessary to establish a method for precise measurement of vancomycin in hospital sewage as an emerging pollutant that causes bacterial resistance. Methods: In this study, solid phase extraction was used for separation of vancomycin from hospital wastewater. Vancomycin recovery rate were investigated at pH 3 and 7. The extracted samples were passed through a C18 column at 20 °C using different ratios of mobile phase of acetate/acetonitrile buffer with flow rate of 0.8 ml min-1. The quantity of vancomycin in samples was detected by high performance liquid chromatography (HPLC) equipped with UV detector at 240 nm wave length. Results: The best condition for extraction was achieved at pH=7 with a recovery of 89%. Better results were obtained by mobile phase of acetate/acetonitrile buffer with volume ratio of 89:11 at pH=4. The linearity coefficients (R2) of 0.97 and 0.96 were obtained for vancomycin concentration ranges of 1-45 and 50-120 µg l-1 at optimum condition, respectively. Limit of Detection (LOD) and Limit of Quantification (LOQ) were calculated as 3 and 10 µg l-1 respectively. Also, variations in precision of both hourly and daily measurements of standard solution were less than 10%. Conclusion: The results of this study might be applied as a specific method for extraction and analysis of vancomycin antibiotic in hospital sewage samples.

Keywords: Vancomycin, Hospital Wastewater, Liquid Chromatography, Solid Phase Extraction.
Full-Text [PDF 889 kb]   (2721 Downloads)    
Type of Study: Research | Subject: Special
Received: 2014/10/22 | Accepted: 2014/10/22 | Published: 2014/10/22
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Safavy S, Ghaemmaghami Hezaveh S, Dehghanzadeh Rayhani R. Extraction and Determination of Vancomycin Antibiotic in Hospital Sewage Samples by Solid Phase Extraction-High Performance Liquid Chromatography. j.health. 2014; 5 (3) :224-234
URL: http://healthjournal.arums.ac.ir/article-1-364-en.html
Volume 5, Issue 3 (autumn 2014) Back to browse issues page
مجله سلامت و بهداشت Journal of Health
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